Tests for the rapid diagnostic of rotavirus english translation. Update on schemes and the future uk neqas microbiology. The study protocol was approved by the institutional ethical. Enteric prevalence of rotavirus and adenovirus in under five children predisposed to acute diarrhea in yaoundecameroon ngum vn. Clostridium difficile infection in polish pediatric outpatients with inflammatory bowel disease d. Detection and molecular characterisation of adenovirus in. Vikia rotaadeno is a rapid, qualitative, chromatographic immunoassay for the simultaneous detection of rotavirus and adenovirus. The elisa test, ideia from dako, considered as the reference method was blindly performed by biomerieux laboratory. Rotavirus epidemiology ods in bangui, central african and. If the sample is adeno positive, we recommend to further confirm the serotype result with 4041 adeno strip. Objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse.
Research tropheryma whipplei in children with gastroenteritis. Ict assay biomerieux, marcy letoile, france, while excellent specificity was. Sensitivity and specificity of the vikia r rotaadeno immunochromatographic test biomerieux and the elisa ideia tm rotavirus kit dako compared to genotyping. Rapid test for the qualitative dual detection of rotavirus and adenovirus in human stools. Interestingly, the specificity of the vikia rotaadeno assay was 100%. Vikia biomerieux, kcombi set bioconnections, rida quick rbiopharm and quickstrip savyon diagnostics. Rotavirus a and adenovirus in samples of human faeces were compared.
We therefore reexamined samples initially testing pos itive in the vikia rotaadeno ict with other commercially available elisa rotavirus assays and, for a subset of specimens, by rtpcr. After receiving concerns regarding this test, company subsequently performed a parallel evaluation of the diagnostic accuracy of vikia rota adeno. Rotavirus infection in children and adult patients. Vikia rota adeno biomerieux diarlex mb fumouze b the two immunochomatographic tests were performed in the clinical laboratory of the debrousse hospital. L, zaragoza, spain and vikia rotaadeno biomerieux sa, lyon, france were retrospectively evaluated using realtime pcr as reference test. Oct 23, 2017 europe pmc is an archive of life sciences journal literature. Fecal rotaadeno duo antigen rapid test kit abin5186. Group a rotaviruses rvas are the primary cause of acute gastroenteritis. We conducted a parallel evaluation of the diagnostic accuracy of vikia rota adeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Acute diarrheal illness was defined as occurrence of.
Tutkimuksen tarkoituksena oli vertailla ka ytossa olevaa diarlex mb vieritestia rida quick ja vikia vieritesteihin. Rotavirus infection in children and adult patients attending in a. The multiplex assays were clinically evaluated using 239 fecal samples submitted to our laboratory over a 1year period for the routine detection of rotavirus andor adenovirus antigens using the vikia rotaadeno test biomerieux, boxtel, the netherlands. Vikia rotaadeno biomerieux, france streptococcus quickvue inline strep a test quidel, usa, and urine dipsticks urilab h10. Adiogo d, fokunang cn, gonsu kh, tembefokunang ea, abena omt. This observation underlines the importance of regular assess. Patientswith ibd were treated with sulfasalazine or mesalazine, azathioprine, cyclosporine, steroids and infliximab according to accepted clinical protocols. Vikia rotaadeno and 10 56 % were positive when analysed by rtpcr table 1. Immunochromatographybased diagnosis of rotavirus infection.
Performance analysis of two immunochromatographic assays. Herve crehalet, francois vandenesch, anne marie freydiere. Adenovirus diagnostic testing market growth analysis 2020 by. We conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Enteric prevalence of rotavirus and adenovirus in under five. Many diagnostic laboratories in australia use the vikia rotaadeno assay for detection of rotavirus in faecal specimens. The samples were analyzed using vikia rotaadeno test kit biometrieux. Accurate detection of rotavirus is essential for prevention and control of rotavirus outbreaks and disease monitoring. For adenovirus, vikia rotaadeno kit produced 1 positive result 5. Detection of rotavirus, norovirus and adenovirus by molecular. Specimens were obtained from 1,350 hospitalized patients and. Acute diarrhea in tropical countries can be caused by a broad spectrum of viral, parasitic and bacterial enteropathogens. The incidence of rotavirus and adenovirus infections among. M dugua 1 introduction results acute gastroenteritis age is one.
Oct 10, 2016 one stool sample was collected for rotavirus testing vikia rotaadeno immunochromatographic test biomerieux from each child sensitivity 96. Enteric prevalence of rotavirus and adenovirus in under. Performance analysis of two immunochromatographic assays for the. Rotavirus infection in children and adult patients attending. After informed consent and epidemiologic and clinical data had been obtained, a fecal specimen was collected from each child. Biomerieux vikia rotaadeno vzporedni test za rotaviruse. Pdf diagnostic accuracy of vikia rotaadeno and premier. Rotavirus epidemiology in bangui, central african republic. A total of 250 infants and young children with diarrhea 159 male and 91 female, mean age 8. Multicenter prospective study on the burden of rotavirus. Results obtained by the vikia rotaadeno test revealed that 100 40% of these children were infected with rotavirusa, mostly male children 61100, p vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Children aged 5 years and under 59% samples had a virus 36. Evaluation of the bionexia rotaadeno rapid test h briand 1, l ji 2, j morgand 1, l thery 3, m bidalot 3, k ambertbalay 3, j kaplon 3 a duraffourgcollomb 1, j. L, zaragoza, spain and vikia rota adeno biomerieux sa, lyon, france were retrospectively evaluated using realtime pcr as reference test.
Biomerieux vikia rotaadeno vzporedni test za rotaviruse in. Diagnostic accuracy of seven commercial assays for rapid detection of group a rotavirus antigens jerome kaplon, aceline fremy, sylvie pillet,b lucile mendes martins,c katia ambertbalay,a serge l. A total of 3,768 stool samples were received during the study period, 55. Pdf sensitivity and specificity of the vikia r rota. Rapid diagnosis of the infectious agent avoids unnecessary antibiotic treatment and leads to rapid isolation of infected patients. Orion diarlex rota microgen rotascreen eia in house pcr biomerieux vikia coris combistrip biokit biorapid novamed rotastick rbiopharm ridaquick rota certest rota adeno card certest rota card atlas medical rota adeno biosigma fast step oxiod prospect quadratech check4 rotavirus rbiopharm ridaquick kombi. Diagnostic accuracy of seven commercial assays for rapid. In the analysis of rotavirus, the molecular and immunological methods had an agreement of 83%, while for adenovirus the agreement.
The full text of this article is available in pdf format. Enteric adenovirus kit pcr positive negative sensitivity % specificity % ppv % npv % vikia positive 8 0 negative 2 190 80 100 100 98. Loinc 803767 rotavirus and adenovirus ag panel stool. This term was created for, but is not limited in use to, the biomerieux vikia rota adeno one step combo card test, which is intended for the dual detection of rotaviruses and adenoviruses in a single stool extract. In our study, the evaluation was done after optimization of the manufacturing process for vikia rotaadeno biomerieux personal communication, which may explain the difference between the australian study and this study.
The aim of this study was to determine the incidence and clinical significance of rotavirus and enteric adenovirus gastroenteritis and also to determine possible risk factors for rotavirus and adenovirus gastroenteritis using new simple rapid screening test vikia rota and adeno. Adenovirus diagnostic testing market growth analysis 2020. Edelleen ripulitauteihin kuolee koko maai lmassa vuosittain 2,5 miljoona lasta. If no stool sample was obtained during the baseline visit, parents were requested to collect a stool sample and bring it into the health care center within four days. Biomerieux vikia rotaadeno vzporedni test za rotaviruse in adenoviruse iz humaneg fecesa bm31111 kratka navodila za rokovanje z instrumentom. Rapid communications evidence of falsepositive results in a.
Vikia rotaadeno is a simple rapid test used as an aid in the immediate diagnosis of viral gastroenteritis by rotaviruses and adenoviruses. Objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rota adeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse. Trends of viral gastroenteritis in the australian capital. In the study, one hundred children less than 5 years of age with acute gastroenteritis admitted to sulaimanipaediatric hospital were studied. Enteric prevalence of rotavirus and adenovirus in under five children predisposed to acute diarrhea in yaounde. Rotavirus epidemiology ods in bangui, central african. The main objective of this study was to identify rotavirus and adenovirus in under five children predisposed with acute diarrhea in yaoundecameroon using vikia rotaadeno rapid test kit biomerieux, france. Comparison of vikia rota adeno to ideia with 189 stool samples.
The vikia rotaadeno test gave a positive result in 24239 10. Sensibilite et specificite du test immunochromatographique vikia. Adeno ja rotaviruksia osoittavien vier i testien vertailu. Vikia rota and adeno biomerieux france is a qualitative test based on the immunochromatography technique ict. All reagents and stool samples were allowed to reach room temperature before use.
Diagnostic accuracy of vikia rotaadeno and premier rotaclone tests for the. Vikia rota adeno kit is a qualitative test based on the association of monoclonal antibodies. Vikia rota adeno is a rapid, qualitative, chromatographic immunoassay for the simultaneous detection of rotaviruses and adenoviruses, which are responsible for the majority of pediatric diarrhea cases. Tests for the rapid diagnostic of rotavirus english. Infection with group a rotavirus is among the lead. Rotavirus was detected every month during the period of the study, with peak of positivity between june and august 35%. Clostridium difficile infection in polish pediatric. From january 2006 to july 2009, 467 samples of faeces from individuals with gastroenteritis. Europe pmc is an archive of life sciences journal literature. The multiplex assays were clinically evaluated using 239 fecal samples submitted to our laboratory over a 1year period for the routine detection of rotavirus andor adenovirus antigens using the vikia rota adeno test biomerieux, boxtel, the netherlands. Detection of rotavirus, norovirus and adenovirus by. Letters rotavirus epidemiology in bangui, central african and vp7 and vp4 were amplified by republic, 20081 to the editor. The pathflow rota adeno combi test cassette is a rapid chromatographic immunoassay for the qualitative detection of rotavirus and adenovirus in human faeces, providing results from 10 minutes offering an easytouse, rapid alternative.
Navodila za uporabo instrumenta naj bodo zmeraj v blizini naprave. We excluded rotavirus specimen tests where age or sex was missing n21. Diagnostic accuracy of vikia rotaadeno and premier. Rapid communications evidence of falsepositive results in. Etiology of febrile illnesses in young children attending.
Performance analysis of two immunochromatographic assays for. Diagnosis of viral gastroenteritis by simultaneous detection. Genotype distribution and phylogenetic analysis of. Pdf sensitivity and specificity of the vikia r rotaadeno. Pred uporabo dobro preberite tudi originalna navodila. Diagnosis of viral gastroenteritis by simultaneous.
During a 24month period from 1 may 2010 adults 15 years with age admitted to one of four hospitals in the central denmark region were examined for rotavirus with vikia rota adeno rapid test in addition to routine culture for bacterial pathogens. M dugua 1 introduction results acute gastroenteritis age is one of the most common diseases in humans and continues to be a cause of high morbidity and mortality in children. Biomerieux vikia rota adeno vzporedni test za rotaviruse in adenoviruse iz humaneg fecesa bm31111 kratka navodila za rokovanje z instrumentom. Serodetection of rotavirus and adenovirus gastroenteritis co. In terms of frequency, rotavirus was detected in10 10% of the samples followed by adenovirus type 4041 44%. Comparison of vikia rotaadeno to ideia with 189 stool samples. Interestingly, the specificity of the vikia rota adeno assay was 100%. Of the 24 hrv positive samples, 19 were confirmed with the multiplex pcr assay and 4 additional positive samples were detected, making a total of 9 discrepant samples. Vikia rotaadeno biomerieux diarlex mb fumouze b the two immunochomatographic tests were performed in the clinical laboratory of the debrousse hospital. The study was part of a rotavirus surveillance project in niger. Feb 17, 2020 the pdf for the study can be requested using the following link. Oct 23, 2017 we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Pdf objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and. This is a qualitative test based on the immunochromatography technique.
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